abs against cyt c  (Proteintech)

Journal: Medical Gas Research

Article Title: ACS6, a Hydrogen sulfide-donating derivative of sildenafil, inhibits homocysteine-induced apoptosis by preservation of mitochondrial function

doi: 10.1186/2045-9912-1-20

Figure Lengend Snippet: Effects of ACS6 on the release of Cyt-c in PC12 cells . After pretreated with 16 μmol/L ACS6 for 30 min, PC12 cells were exposed to 5 mmol/L homocysteine (Hcy) for 24 h. A, representative immunoblots for Cyt-c release from three independent experiments. Cytosolic fractions of the extract were subjected to Western blot analysis using an anti-Cyt-c antibody. In all blots, staining for β-actin was used as a loading control. B, quantification of cytosolic Cyt-c accumulation as a percent of the control. Values are the mean ± SEM ( n = 3). *** P < 0.001, versus control; # P < 0.05, versus 5 mmol/L homocysteine-treated alone group.

Article Snippet: Antibody against cytochrome c (Cyt-c) was purchased from Abcam Technology (Cambridge, CB, UK).

Techniques: Western Blot, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway

doi: 10.1155/2017/3514914

Figure Lengend Snippet: Expressions of cyt c and Smac, cleaved caspase-3, and bcl-2. (a) for Western blotting images; (b) for cyt c in different groups; (c) for Smac; (d) for cleaved caspase-3; (e) for bcl-2. The bar graph shows the ratio of the cyt c, Smac, cleaved caspase-3, and bcl-2 integrated density value (IDV) to GAPDH IDV for each experimental condition. Data represent the mean ± SD of 3 groups ( n = 5 in each group). As figures showed, the increase of cytoplasmic cyt c and Smac took place after SAH and it was significantly inhibited by TMP treatment. Sham for sham-operation group; SAH for subarachnoid hemorrhage; TMP for tetramethylpyrazine; GAPDH for glyceraldehyde 3-phosphate dehydrogenase; cyt c for cytochrome c; Smac for second mitochondria-derived activator of caspases; bcl-2 for B-cell lymphoma 2. ( ∗ P < 0.05 compared with the sham-operation group, # P < 0.05 compared with the SAH + Vehicle group.)

Article Snippet: After being blocked with 5% nonfat milk for 90 minutes at room temperature, the sheets were incubated overnight at 4°C with primary antibodies against cyt c (1 : 1000, Abcam, UK), cleaved caspase-3 (1 : 500, Cell Signaling Technology, USA), bcl-2 (1 : 1000, Santa Cruz Biotech, USA), Smac (1 : 1000, Cell Signaling Technology, USA), and the loading control GAPDH (1 : 5000, Abcam, UK).

Techniques: Western Blot, Derivative Assay